Objective: To investigate the suppression effect and apoptosis of the colon cell line HT-29 treated with adenovirus-expressed human hyperplasia suppressor gene (hHSG) and hyperthermia.
Methods: Replication-defective recombinant adenovirus containing hHSG was constructed, and then infected into HT-29 cell line to detect its overexpression by western blot. The synergic effect with hyperthermia was observed, cell survival was assessed using Vi-CELL TMXR Cell Viability Analyzer, and cell apoptosis and cell cycle distribution were determined by flow cytometry.
Results: After the cells were treated with 100 MOI (multiplicity of infection) Ad5-hHSG and heated at 42 degree C for 1 h, the number of viable cells decreased sharply, compared with single treatment with Ad5-hHSG or 42 degree C for 1 h (P < 0.01). The apoptotic rate of combined groups was significantly higher than that of Ad5-hHSG group or the heated group respectively (P < 0.01). Moreover, the combination treatment of Ad5-hHSG and 42 degree C for 1 h induced G2/M arrest.
Conclusion: Ad5-hHSG could increase the sensitivity of HT-29 to hyperthermia.