Activation of class II gene transcription by regulatory factors is potentiated by a novel activity

Cell. 1991 Sep 6;66(5):981-93. doi: 10.1016/0092-8674(91)90443-3.

Abstract

A novel activity (USA) stimulated activator-dependent transcription in a reconstituted system in conjunction with natural TFIID, resulting in 10- to 50-fold levels of induction by regulatory factors. USA mediated a modest induction by USF in conjunction with either recombinant human TFIID, intact yeast TFIID, or the evolutionarily conserved C-terminal portion of yeast TFIID. Upon further purification, USA was resolved into two components that had opposite effects on core promoter activity and that in combination potentiated activator function. Gel mobility shift experiments indicated physical interactions between the inhibitory activity and TFIID, suggesting that the additional components (cofactors) associate with the preinitiation complex both to reduce promoter activity in the absence and to increase promoter activity in the presence of transcriptional activators.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviruses, Human / genetics
  • DNA-Binding Proteins / physiology
  • Gene Expression Regulation*
  • HIV-1 / genetics
  • HeLa Cells
  • Humans
  • In Vitro Techniques
  • Promoter Regions, Genetic*
  • Regulatory Sequences, Nucleic Acid
  • Transcription Factors / physiology*
  • Transcription, Genetic*
  • Transcriptional Activation

Substances

  • DNA-Binding Proteins
  • Transcription Factors