Aptabodies - new type of artificial receptors for detection proteins

Protein Pept Lett. 2008;15(8):799-805. doi: 10.2174/092986608785203656.

Abstract

We report on a new type of artificial receptor formed by hybridization of two DNA aptamers for human thrombin (aptabody). This aptasensor based on multiwalled carbon nanotubes allowed us to detect thrombin with detection limit of 0.3 nM, which was 3 times better in comparison with conventional aptamer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aptamers, Nucleotide / chemistry
  • Aptamers, Nucleotide / genetics*
  • Aptamers, Nucleotide / metabolism*
  • Base Sequence
  • Biosensing Techniques / methods*
  • Biotinylation
  • Electrochemistry
  • Humans
  • Methylene Blue / metabolism
  • Nanotubes, Carbon
  • Nucleic Acid Conformation
  • Nucleic Acid Hybridization
  • Pancreatic Elastase / metabolism
  • Sensitivity and Specificity
  • Serum Albumin / metabolism
  • Thrombin / metabolism*

Substances

  • Aptamers, Nucleotide
  • Nanotubes, Carbon
  • Serum Albumin
  • Pancreatic Elastase
  • Thrombin
  • Methylene Blue