Objective: To make qualitative analysis on alkaliod in Lobelia sessilifolia.
Method: The ESI-MS(n) method was used for qualitative analysis.
Result: The structures of two alkaliods in plant extract of L. sessilifolia were successfully analyzed and identified as norlobelanine and 8, 10-diethyllobelidione by electrospray ionization multistage mass spectrometry in positive mode. Then the fragmentation mechanisms were explored.
Conclusion: SPE and ESI-MS(n) could simply and rapidly provide qualitative examination of alkaliods in L. sessilifolia.