Objective: To identify dihydroflavonol glycoside isomers in Smilax glabra.
Method: The sample was analyzed by HPLC-MS in combination with HPLC-1H-NMR.
Result: Four dihydroflavonol glycoside isomers were identified as astilbin, neoastilbin, isoastilbin, neoisoastilbin.
Conclusion: The method is simple and rapid for the identification of dihydroflavonol glycosides in S. glabra.