Abstract
Bioactivity-guided fractionation of Corydalis yanhusuo has resulted in the isolation of eight known isoquinoline alkaloids - tetrahydropalmatine, isocorypalmine, stylopine, corydaline, columbamine, coptisin, 13-methylpalmatine, and dehydrocorybulbine. The tertiary alkaloids were further analyzed by chiral HPLC to determine the ratios of d-and l-isomers. The isolated compounds were screened for their binding affinities at the dopamine D(1) receptor. Isocorypalmine had the highest affinity (K(i) = 83 nM). The structure-affinity relationships of these alkaloids are discussed.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Alkaloids / chemistry*
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Alkaloids / isolation & purification
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Alkaloids / metabolism
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Berberine Alkaloids / chemistry*
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Berberine Alkaloids / isolation & purification
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Berberine Alkaloids / metabolism
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Binding, Competitive
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Cell Line
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Chromatography, High Pressure Liquid
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Corydalis / chemistry*
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Humans
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Isoquinolines / chemistry*
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Isoquinolines / isolation & purification
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Isoquinolines / metabolism
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Molecular Structure
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Plant Extracts / chemistry
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Receptors, Dopamine D1 / genetics
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Receptors, Dopamine D1 / metabolism*
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Structure-Activity Relationship
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Transfection
Substances
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Alkaloids
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Berberine Alkaloids
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Isoquinolines
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Plant Extracts
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Receptors, Dopamine D1
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isocorypalmine
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corydaline
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tetrahydropalmatine
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columbamine
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stylopine
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isoquinoline