A potyvirus was isolated from Brugmansia suaveolens showing leaf mottling and tentatively named Brugmansia suaveolens mottle virus (BsMoV). The virus (isolate Bs-Campinas) could infect some solanaceous plants and two Chenopodium species, and was transmitted by aphids. Symptomatic leaves contained flexuous particles and cylindrical inclusions. RT-PCR amplification using potyvirus universal primers produced a DNA fragment of 1851 nt (3' terminal genomic region), which shared 71% nucleotide identity with Pepper mottle virus, the best-matched potyvirus sequence. Since this identity value is below the threshold currently used to discriminate Potyvirus species, Brugmansia suaveolens mottle virus most likely represents a new Potyvirus species.