Abstract
Sedum telephium ssp. maximum is a medicinal plant that possesses anti-inflammatory, analgesic and keratolytic properties. We investigated the anti-inflammatory activity of its methanolic extract (STME) in rat peritoneal macrophages (MPhis) stimulated with lipopolysaccharide from Salmonella enteritidis. After stimulation with 10 microg/ml of LPS, MPhis were coincubated with different doses of STME (8, 16 and 32 microg/ml) or RPMI medium alone using different times of incubation. STME reduced levels of tumor necrosis factor-alpha, both mRNA and its protein, and significantly decreased IL-1beta and IL-6 production. Moreover, STME inhibited inducible nitric oxide synthase expression and blunted nitrite release and inhibited both extracellular signal-regulated kinase 1/2 and c-Jun N-terminal kinase activation in lipopolysaccharide-stimulated MPhis. Data show that STME might be useful as a potential anti-inflammatory agent.
Copyright 2008 S. Karger AG, Basel.
MeSH terms
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Animals
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Anti-Inflammatory Agents / administration & dosage
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Dose-Response Relationship, Drug
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Gene Expression Regulation / drug effects
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Inflammation / drug therapy*
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Inflammation / physiopathology
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Interleukin-1beta / drug effects
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Interleukin-1beta / metabolism
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Interleukin-6 / metabolism
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JNK Mitogen-Activated Protein Kinases / drug effects
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JNK Mitogen-Activated Protein Kinases / metabolism
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Lipopolysaccharides
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Macrophages, Peritoneal / drug effects
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Macrophages, Peritoneal / metabolism
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Male
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Mitogen-Activated Protein Kinase 1 / drug effects
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Mitogen-Activated Protein Kinase 1 / metabolism
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Mitogen-Activated Protein Kinase 3 / drug effects
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Mitogen-Activated Protein Kinase 3 / metabolism
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Nitric Oxide Synthase Type II / drug effects
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Nitric Oxide Synthase Type II / metabolism
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Plant Extracts / administration & dosage
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Plant Extracts / pharmacology*
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RNA, Messenger / drug effects
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Salmonella enteritidis / immunology
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Sedum / chemistry*
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Tumor Necrosis Factor-alpha / drug effects
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Anti-Inflammatory Agents
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Interleukin-1beta
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Interleukin-6
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Lipopolysaccharides
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Plant Extracts
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Nitric Oxide Synthase Type II
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3