[DNA polymorphism detection of Papaver somniferum L using fluorescent amplified fragment length polymorphism]

Fa Yi Xue Za Zhi. 2008 Aug;24(4):262-4, 267.
[Article in Chinese]

Abstract

Objective: To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.

Methods: Genomic DNA was isolated using the AxyPrep DNA Kit, double-digested by two restrictional endonucleases (Eco RI and Mse I) and ligated to oligonucleotide adapters. After Pre-amplification and selective amplification, the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.

Results: More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.

Conclusion: The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amplified Fragment Length Polymorphism Analysis / methods*
  • DNA, Plant / analysis
  • DNA, Plant / genetics*
  • Fluorescent Dyes
  • Forensic Genetics
  • Papaver / genetics*
  • Polymorphism, Genetic*

Substances

  • DNA, Plant
  • Fluorescent Dyes