Assessment of liver phagocytic activity using EPR spectrometry and imaging

Nicolas Charlier; Audrey M Neyrinck; Nelson Beghein; Nathalie M Delzenne; Bernard Gallez

doi:10.1016/j.mri.2008.07.022

Assessment of liver phagocytic activity using EPR spectrometry and imaging

Magn Reson Imaging. 2009 May;27(4):565-9. doi: 10.1016/j.mri.2008.07.022. Epub 2008 Sep 20.

Authors

Nicolas Charlier¹, Audrey M Neyrinck, Nelson Beghein, Nathalie M Delzenne, Bernard Gallez

Affiliation

¹ Biomedical Magnetic Resonance Unit, Louvain Drug Research Institute, Université catholique de Louvain, 1200 Brussels, Belgium.

PMID: 18805665
DOI: 10.1016/j.mri.2008.07.022

Abstract

The aim of the present study was to evaluate the usefulness of electron paramagnetic resonance (EPR) spectroscopy and imaging in assessing the phagocytic activity of the liver after administration of India ink. We conducted experiments on livers from control rodents and from rodents in which the Kupffer cell population had been depleted by pretreatment with gadolinium chloride. The EPR signal intensity recorded in liver homogenates was about two times lower in GdCl(3) treated rats than in control rats. EPR imaging carried out on precision-cut liver slices indicated a good correlation between the depletion of Kupffer cells and the EPR signal intensity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Electron Spin Resonance Spectroscopy / methods*
Image Enhancement / methods
Image Interpretation, Computer-Assisted / methods*
Liver / cytology*
Liver / physiology*
Magnetic Resonance Imaging / methods*
Male
Phagocytosis / physiology*
Rats
Rats, Wistar
Reproducibility of Results
Sensitivity and Specificity