Abstract
Taking advantage of a mutant estrogen receptor ligand binding domain (ER(T2)), we developed novel Caspase fusion proteins for inducible apoptosis. We show that Caspase-ER(T2) fusion proteins become specifically activated by the synthetic ligand 4-OH- tamoxifen and rapidly induce apoptotic cell death in human, murine, and zebrafish cells. This novel tool for targeted cell ablation greatly facilitates the generation of disease models as well as developmental and regeneration studies in model organisms.
Copyright 2008 Wiley-Liss, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents, Hormonal / pharmacology*
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Apoptosis / drug effects*
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Apoptosis / genetics
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Binding Sites / drug effects
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Binding Sites / genetics
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Caspases / genetics
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Caspases / physiology*
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Cell Line
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Cell Line, Transformed
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Genes, Transgenic, Suicide* / drug effects
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HeLa Cells
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Humans
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Mice
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Selective Estrogen Receptor Modulators / pharmacology
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Tamoxifen / pharmacology*
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Zebrafish
Substances
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Antineoplastic Agents, Hormonal
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Selective Estrogen Receptor Modulators
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Tamoxifen
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Caspases