Chemoenzymatic synthesis of GDP-azidodeoxymannoses: non-radioactive probes for mannosyltransferase activity

Silvia Marchesan; Derek Macmillan

doi:10.1039/b807016d

Chemoenzymatic synthesis of GDP-azidodeoxymannoses: non-radioactive probes for mannosyltransferase activity

Chem Commun (Camb). 2008 Sep 28:(36):4321-3. doi: 10.1039/b807016d. Epub 2008 Jul 18.

Authors

Silvia Marchesan¹, Derek Macmillan

Affiliation

¹ Department of Chemistry, University College London, 20 Gordon Street, London, UK WC1H 0AJ.

PMID: 18802557
DOI: 10.1039/b807016d

Abstract

GDP-2-, 3-, 4- or 6-azidomannoses can be successfully prepared from the corresponding azidomannose-1-phosphates and GTP using the enzyme GDP-Mannosepyrophosphorylase (GDP-ManPP) from Salmonella enterica and may serve as useful probes for mannosyltransferase activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation
Enzyme Activation
Guanosine Diphosphate Mannose / analogs & derivatives*
Guanosine Diphosphate Mannose / chemical synthesis*
Guanosine Diphosphate Mannose / chemistry
Guanosine Triphosphate / chemistry
Mannosyltransferases / analysis
Mannosyltransferases / chemistry*
Molecular Probes / chemistry*
Recombinant Proteins / chemistry
Salmonella enterica / enzymology

Substances

Molecular Probes
Recombinant Proteins
Guanosine Diphosphate Mannose
Guanosine Triphosphate
Mannosyltransferases