URPOSE: To isolate and culture dental pulp stem cells from human deciduous teeth, and induce them to osteoblasts.
Methods: Dental pulp stem cells were separated from deciduous teeth with enzyme digestionìthen isolated and purified by limited dilution. The cells clone form rate was carried out, and cell growth curve was measured by counting the number of cells. HE stain and immunhistochemistry stain of VimentinìCD44 and STRO-1 were tested. Then the cells were induced to osteoblasts, and HE stain,AKP stain, Von Kossa stain,Van Gieson stain,and immunhistochemistry stain of osteocalcin were used for evaluation.
Results: Dental pulp stem cells were obtained from deciduous teeth by limited dilution. The cells were induced to osteoblasts, which elicited a biological and morphologic characteristics similar to those of osteoblasts.
Conclusions: The results show that dental pulp stem cells are obtained from deciduous teeth and induced to osteoblasts successfully.