(N-succinimidyl 4-pentynoate)hexacarbonyldicobalt: a transition-metal carbonyl complex having similar uses to the Bolton-Hunter reagent

M Salmain; A Vessières; I S Butler; G Jaouen

doi:10.1021/bc00007a002

(N-succinimidyl 4-pentynoate)hexacarbonyldicobalt: a transition-metal carbonyl complex having similar uses to the Bolton-Hunter reagent

Bioconjug Chem. 1991 Jan-Feb;2(1):13-5. doi: 10.1021/bc00007a002.

Authors

M Salmain¹, A Vessières, I S Butler, G Jaouen

Affiliation

¹ Ecole Nationale Supérieure de Chimie de Paris, U.A. C.N.R.S 403, France.

PMID: 1878407
DOI: 10.1021/bc00007a002

Abstract

The synthesis of the transition-metal carbonyl complex (N-succinimidyl 4-pentynoate)hexacarbonyldicobalt [[(C4H4O2N)O(CO)CH2CH2C identical to CH]Co2(CO)6] is described. This cobalt carbonyl complex is structurally similar to the Bolton-Hunter conjugation reagent and has been successfully employed as a nonradioactive tracer for labeling the drug carbamazepine. The metal carbonyl tracer can be detected at extremely low concentrations (ca. 1 pmol) by FT-IR spectroscopy in the v(CO) region (2150-1800 cm-1). The cobalt carbonyl labeled carbamazepine retains good recognition for anti-carbamazepine antibodies. This novel labeling procedure, which can be broadly termed carbonylmetalloimmunoassay (CMIA), has considerable potential for assaying a wide range of biological materials.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cobalt / chemistry
Organometallic Compounds / chemistry*
Succinimides / chemistry*

Substances

Organometallic Compounds
Succinimides
(N-succinimidyl 4-pentynoate)hexacarbonyldicobalt
Cobalt
Bolton-Hunter reagent