In vitro selection of a DNAzyme with three modified nucleotides

Marcel Hollenstein; Christopher Hipolito; Curtis Lam; David Perrin

doi:10.1093/nass/nrn037

In vitro selection of a DNAzyme with three modified nucleotides

Nucleic Acids Symp Ser (Oxf). 2008:(52):73-4. doi: 10.1093/nass/nrn037.

Authors

Marcel Hollenstein¹, Christopher Hipolito, Curtis Lam, David Perrin

Affiliation

¹ Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver BC, V6T 1Z1, Canada.

PMID: 18776259
DOI: 10.1093/nass/nrn037

Abstract

Three modified nucleosides have been used in the in vitro selection of the self-cleaving DNAzyme 9-33. This DNAzyme operates in the absence of divalent metal cations with a first-order constant of approximately 0.05 min(-1).

MeSH terms

Base Sequence
Catalysis
DNA, Catalytic / chemistry*
Molecular Sequence Data
Nucleotides / chemistry*

Substances

DNA, Catalytic
DNAzyme 9-33
Nucleotides