Isolation of small RNAs using biotinylated PNAs

J Biochem. 2008 Oct;144(4):415-8. doi: 10.1093/jb/mvn107. Epub 2008 Sep 3.

Abstract

In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from human total RNA in pure form. Damage to the RNA appears to be negligible by this method because the method is rapid and does not require a high temperature treatment to facilitate RNA-PNA binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Biotinylation
  • Cattle
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / chemistry
  • Escherichia coli / genetics
  • Humans
  • Methods
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Peptide Nucleic Acids / chemistry
  • RNA / chemistry
  • RNA / isolation & purification*
  • RNA, Bacterial / chemistry
  • RNA, Bacterial / genetics
  • RNA, Bacterial / isolation & purification
  • RNA, Transfer, Leu / chemistry
  • RNA, Transfer, Leu / genetics
  • RNA, Transfer, Leu / isolation & purification
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Peptide Nucleic Acids
  • RNA, Bacterial
  • RNA, Transfer, Leu
  • RNA