SNP analysis is one of the most contemporary methods for personal identification in forensic genetics. It is increasingly more frequently used in forensic practice, especially for analyses of highly degraded DNA samples from crime scenes and thus it requires suitable population data. The aim of this study was to develop a central Poland population database consisting of 500 alleles in a range of 5 SNP biallelic loci (rs2294067, rs2282160, rs2070764, rs2277216, rs1063739). DNA fragments were amplified in one multiplex PCR reaction and SNPs were identified in a minisequencing reaction. The combined PD of the pentaplex was 0.9907147657. This makes the pentaplex a good screening method for forensic applications.