Abstract
Escherichia coli failed to survive after transformation with a Streptococcus intermedius histone-like protein gene (Si-hlp) and its promoter-harbored plasmid. The promoter function of Si-hlp in E. coli was determined using enhanced green fluorescence protein (egfp) gene as a reporter. The inhibitory effect of Si-HLP on E. coli viability was verified by a tetracycline-inducible gene expression system. Further study suggested that Si-HLP may alter the bacterial nucleoid structure, leading to the growth inhibition of E. coli.
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Cell Nucleolus / chemistry*
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Cell Nucleolus / genetics
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Cell Nucleolus / metabolism
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Cloning, Molecular
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Superhelical / chemistry
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DNA, Superhelical / genetics
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Escherichia coli / chemistry
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Escherichia coli / genetics
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Escherichia coli / growth & development*
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Escherichia coli / metabolism
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Gene Expression*
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Histones / genetics
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Histones / metabolism*
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Streptococcus intermedius / metabolism*
Substances
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Bacterial Proteins
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DNA, Bacterial
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DNA, Superhelical
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Histones