Abstract
The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5alpha as a 6x His-tagged protein. The purified 6x His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology*
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Antibody Specificity
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Blotting, Western
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Cattle
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Escherichia coli / genetics
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Female
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Humans
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Hybridomas / immunology
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Immunohistochemistry
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Mice
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Recombinant Fusion Proteins / analysis
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Recombinant Fusion Proteins / immunology
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Recombinant Fusion Proteins / metabolism
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Recombinant Fusion Proteins / pharmacokinetics
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Staining and Labeling
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Tissue Distribution
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Tumor Suppressor Proteins / analysis
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Tumor Suppressor Proteins / immunology*
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Tumor Suppressor Proteins / metabolism
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Tumor Suppressor Proteins / pharmacokinetics
Substances
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Antibodies, Monoclonal
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NDRG2 protein, human
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Recombinant Fusion Proteins
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Tumor Suppressor Proteins