Simultaneous multi-parameter observation of Harringtonine-treating HL-60 cells with both two-photon and confocal laser scanning microscopy

C Zhang; Y Li; H Ma; S Li; S Xue; D Chen

doi:10.1007/BF02879605

Simultaneous multi-parameter observation of Harringtonine-treating HL-60 cells with both two-photon and confocal laser scanning microscopy

Sci China C Life Sci. 2001 Aug;44(4):383-91. doi: 10.1007/BF02879605.

Authors

C Zhang¹, Y Li, H Ma, S Li, S Xue, D Chen

Affiliation

¹ Department of Physics, Tsinghua University, Beijing, China.

PMID: 18726419
DOI: 10.1007/BF02879605

Abstract

Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluorescent probe Hoechst 33342, tetramethyrhodamine ethyl ester (TMRE) and Fluo 3-AM, we simultaneously observed HT-induced changes in nuclear morphology, mitochondrial membrane potential and intracellular calcium concentration ([Ca(2+)](i)) in HL-60 cells, and developed a real-time, sensitive and invasive method for simultaneous multi-parameter observation of drug-treating living cells at the level of single cell.