Tissue-specific expression and post-translational modification of histone H3 variants

J Proteome Res. 2008 Oct;7(10):4225-36. doi: 10.1021/pr800044q. Epub 2008 Aug 14.

Abstract

Analyses of histone H3 from 10 rat tissues using a Middle Down proteomics platform revealed tissue-specific differences in their expression and global PTM abundance. ESI/FTMS with electron capture dissociation showed that, in general, these proteins were hypomodified in heart, liver and testes. H3.3 was hypermodified compared to H3.2 in some, but not all tissues. In addition, a novel rat testes-specific H3 protein was identified with this approach.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Histones / genetics
  • Histones / metabolism*
  • Humans
  • Lysine / metabolism
  • Male
  • Mass Spectrometry / methods
  • Molecular Sequence Data
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism*
  • Protein Processing, Post-Translational*
  • Rats
  • Rats, Sprague-Dawley
  • Testis / metabolism
  • Tissue Distribution

Substances

  • Histones
  • Protein Isoforms
  • Lysine