The test-retest reliability of the auditory steady-state response (ASSR) has received limited attention. Therefore, the aim was to assess the test-retest reliability of an 80-Hz multiple-ASSR system in normal-hearing subjects by a comprehensive set of statistical methods. Twenty-nine participants (15 females) aged between 18 and 30 years contributed to two sessions (test-retest), and the ASSR thresholds were determined with a descending search protocol using a 10-dB precision. The test-retest reliability was assessed by a three-layered approach which consisted of Pearson product-moment correlation, analysis of variance (ANOVA), and standard error of measurement (SEM). The correlations for ASSR thresholds ranged from poor (0.34) for 500 Hz CF to moderate (0.55) for 1000, 2000, and 4000 Hz CF. A two-way ANOVA of the difference scores (ASSR threshold minus behavioral threshold) demonstrated no significant difference between test and retest. The SEM determined the normal tolerance for clinical error of repeated thresholds and the ASSR SEM values fell well within +/-10 dB HL. This investigation shows that the multiple ASSR-technique produces a clinically acceptable test-retest reliability for normal-hearing adults.