A MALDI TOF MS-based minisequencing method for rapid detection of TEM-type extended-spectrum beta-lactamases in clinical strains of Enterobacteriaceae

L N Ikryannikova; E A Shitikov; D G Zhivankova; E N Il'ina; M V Edelstein; V M Govorun

doi:10.1016/j.mimet.2008.07.005

A MALDI TOF MS-based minisequencing method for rapid detection of TEM-type extended-spectrum beta-lactamases in clinical strains of Enterobacteriaceae

J Microbiol Methods. 2008 Dec;75(3):385-91. doi: 10.1016/j.mimet.2008.07.005. Epub 2008 Jul 16.

Authors

L N Ikryannikova¹, E A Shitikov, D G Zhivankova, E N Il'ina, M V Edelstein, V M Govorun

Affiliation

¹ Research Institute for Physical-Chemical Medicine of Ministry of Public Health of Russian Federation, Malaya Pirogovskaya 1a, Moscow, Russia. larisa@kge.msu.ru

PMID: 18694787
DOI: 10.1016/j.mimet.2008.07.005

Abstract

A minisequencing method based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) was developed for rapid identification of single nucleotide polymorphisms at bla(TEM) gene codons 104, 164 and 238 associated with extended-spectrum activity on TEM-type beta-lactamases. The method was validated by testing the Escherichia coli and Klebsiella pneumoniae strains possessing the known bla(TEM) gene sequences.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Base Sequence
Drug Resistance, Bacterial
Enterobacteriaceae / enzymology
Enterobacteriaceae / genetics
Enterobacteriaceae / isolation & purification*
Enterobacteriaceae Infections / microbiology*
Escherichia coli / genetics
Humans
Klebsiella pneumoniae / genetics
Molecular Sequence Data
Polymorphism, Single Nucleotide
Sequence Analysis, DNA / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Bacterial Proteins
beta-Lactamases