A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis

Chun Yi Liau; Chung-Sheng Lin

doi:10.1263/jbb.106.111

A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis

J Biosci Bioeng. 2008 Jul;106(1):111-3. doi: 10.1263/jbb.106.111.

Authors

Chun Yi Liau¹, Chung-Sheng Lin

Affiliation

¹ Institute of Medicine, Chung Shan Medical University, Taichung 402, Taiwan, R.O.C.; Department of Bioengineering, Tatung University, Taipei 104, Taiwan, ROC.

PMID: 18691542
DOI: 10.1263/jbb.106.111

Abstract

A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme. Using Streptomyces griseus chitinase, we have demonstrated that our method is more sensitive and less complicated than the conventional Calcofluor white M2R staining.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chitinases / analysis*
Chitinases / chemistry*
Electrophoresis, Polyacrylamide Gel / methods
Enzyme Activation
Molecular Weight
Rosaniline Dyes / chemistry*
Staining and Labeling / methods

Substances

Rosaniline Dyes
Coomassie brilliant blue R
Chitinases