Polymerase chain reaction-based biochemical logic gate coupled with cell-free transcription-translation of green fluorescent protein as a report gate

Chem Commun (Camb). 2008 Aug 28:(32):3771-3. doi: 10.1039/b807039c. Epub 2008 Jun 17.

Abstract

Polymerase chain reaction-based biochemical logic gates were designed for AND, OR, NOT, and AND-NOT operations, whose output signal is reported by coupled cell-free transcription-translation of green fluorescent protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell-Free System
  • DNA
  • Green Fluorescent Proteins / genetics*
  • Microfluidics
  • Protein Biosynthesis / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Transcription, Genetic / physiology*

Substances

  • Green Fluorescent Proteins
  • DNA