Characterization of spirostanol saponins in Solanum torvum by high-performance liquid chromatography/evaporative light scattering detector/electrospray ionization with multi-stage tandem mass spectrometry

Rapid Commun Mass Spectrom. 2008 Aug;22(16):2447-52. doi: 10.1002/rcm.3630.

Abstract

High-performance liquid chromatography with an evaporative light scattering detector and electrospray ionization multistage tandem mass spectrometry (HPLC/ELSD/ESI-MS(n)) was used to identify spirostanol saponins in a saponin extract of Solanum torvum. The fragmentation behavior of saponins was studied using ESI-MS(1-3) in positive ion mode. The MS(n) spectra of the [M+H](+) ions provide structural information including aglycone type and the nature and sequence of sugars. The use of ELSD allowed the profiling of the nonchromophore-containing saponins in this plant. The MS analysis established in this study with known saponins was successfully applied to tentatively identify two new siprostanol glycosides, neosolaspigenin 6-O-beta-D-quinovopyranoside and solagenin 6-O-[beta-D-xylopyranosyl-(1 --> 3)-O-beta-D-quinovopyranoside].

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Plant Extracts / chemistry
  • Plants, Medicinal*
  • Saponins / chemistry*
  • Scattering, Radiation
  • Solanum / chemistry*
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Spirostans / chemistry*
  • Tandem Mass Spectrometry / methods*

Substances

  • Plant Extracts
  • Saponins
  • Spirostans