Abstract
Earlier reports found that calsenilin is a transcriptional repressor or a subunit of plasma membrane channel, and indicated that calsenilin was present in the nucleus or plasma membrane. Immunohistochemical and subcellular fractionation analysis, however, revealed that calsenilin/DREAM/KChIP3 was distributed throughout the cytoplasm of SK-N-BE2(C), Jurkat, and HeLa cells. In addition, the expression of calsenilin suppressed the ATP-induced increase in intracellular Ca2+ concentrations. By increase in intracellular calcium concentration, calsenilin was translocated into the nucleus.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
COS Cells
-
Calcium / analysis
-
Calcium / metabolism*
-
Cell Fractionation / methods
-
Cell Line, Tumor
-
Cell Nucleus / metabolism*
-
Chlorocebus aethiops
-
Green Fluorescent Proteins / genetics
-
Green Fluorescent Proteins / metabolism
-
HeLa Cells
-
Humans
-
Immunohistochemistry
-
Intracellular Space / metabolism
-
Jurkat Cells
-
Kv Channel-Interacting Proteins / genetics
-
Kv Channel-Interacting Proteins / metabolism*
-
Microscopy, Fluorescence
-
Plasmids / genetics
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
Repressor Proteins / genetics
-
Repressor Proteins / metabolism*
-
Subcellular Fractions / metabolism
Substances
-
KCNIP3 protein, human
-
Kv Channel-Interacting Proteins
-
Recombinant Fusion Proteins
-
Repressor Proteins
-
Green Fluorescent Proteins
-
Calcium