Kinetics study on a novel natural inhibitor of alpha-chymotrypsin

Viqar Uddin Ahmad; Muhammad Arif Lodhi; Muhammad Athar Abbasi; Muhammad Iqbal Choudhary

doi:10.1016/j.fitote.2008.05.004

Kinetics study on a novel natural inhibitor of alpha-chymotrypsin

Fitoterapia. 2008 Dec;79(7-8):505-8. doi: 10.1016/j.fitote.2008.05.004. Epub 2008 Jun 22.

Authors

Viqar Uddin Ahmad¹, Muhammad Arif Lodhi, Muhammad Athar Abbasi, Muhammad Iqbal Choudhary

Affiliation

¹ Dr. Panjwani Center for Molecular Medicine and Drug Research, HEJ Research Institute of Chemistry, International Centre for Chemical Sciences, University of Karachi, Karachi-75270, Pakistan. vuahmad@cyber.net.pk

PMID: 18620032
DOI: 10.1016/j.fitote.2008.05.004

Abstract

The mechanism of inhibition of alpha-chymotrypsin by benzoylsalireposide (1), a phenolic glycoside isolated from Symplocos racemosa, was investigated. Lineweaver-Burk and Dixon plots and their secondary replots showed that 1 was a non-competitive inhibitor of this enzyme with the Ki value of 29.60 microM.

MeSH terms

Chymotrypsin / antagonists & inhibitors*
Glycosides / chemistry
Glycosides / isolation & purification
Glycosides / pharmacokinetics*
Glycosides / pharmacology
Magnoliopsida / chemistry*
Molecular Structure
Plant Extracts / chemistry*
Serine Proteinase Inhibitors / chemistry
Serine Proteinase Inhibitors / isolation & purification
Serine Proteinase Inhibitors / pharmacokinetics*
Serine Proteinase Inhibitors / pharmacology

Substances

Glycosides
Plant Extracts
Serine Proteinase Inhibitors
benzoylsalireposide
Chymotrypsin
alpha-chymotrypsin