Abstract
The present study aimed to assess whether a newly constructed, catalase-deficient Escherichia coli strain that express mammalian catalase gene could be used to identify oxidative stress-generating chemicals. We tested l-3,4-dihydroxyphenylalanine (l-DOPA), a well-known agent that induces reactive oxygen species. We found that l-DOPA exposure reduced the survival of catalase-mutant E. coli in a dose-dependent manner, especially in the strains with lower catalase activities, implying the usefulness of these strains in assessment of oxidative chemicals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Biological Assay / methods*
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Catalase / genetics*
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Dihydroxyphenylalanine / toxicity*
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Dose-Response Relationship, Drug
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Environmental Monitoring / instrumentation*
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Environmental Monitoring / methods
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Escherichia coli / drug effects*
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Levodopa / pharmacology
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Mutation
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Oxidative Stress
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Reactive Oxygen Species
Substances
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Bacterial Proteins
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Reactive Oxygen Species
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Levodopa
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Dihydroxyphenylalanine
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Catalase