Fluorescence multicolor hologram recorded by using a macrolens array

Opt Lett. 2008 Jul 1;33(13):1461-3. doi: 10.1364/ol.33.001461.

Abstract

We present a new efficient method for obtaining multicolor digital Fresnel holograms of three-dimensional (3-D) objects emitting incoherent fluorescent light. For each emitted fluorescent light wavelength, a single monochromatic digital modified Fresnel hologram of the 3-D scene is generated. Using a macrolens array, only nine projections of the 3-D scene are captured in a single camera shot and are then digitally processed to yield the hologram. The 3-D reconstructed images from all the monochromatic holograms are composed into a single multicolor image of the 3-D scene. The proposed holographic technique is demonstrated by experiments.

MeSH terms

  • Colorimetry / instrumentation*
  • Colorimetry / methods
  • Equipment Design
  • Equipment Failure Analysis
  • Holography / instrumentation*
  • Image Enhancement / instrumentation*
  • Image Interpretation, Computer-Assisted / instrumentation*
  • Imaging, Three-Dimensional / instrumentation*
  • Lenses*
  • Microscopy, Fluorescence, Multiphoton / instrumentation*
  • Microscopy, Fluorescence, Multiphoton / methods
  • Reproducibility of Results
  • Sensitivity and Specificity