Abstract
Deoxypodophyllotoxin (DPT), a naturally occurring flavolignan with anti-inflammatory activity, was isolated from Anthriscus sylvestris HOFFM., and we examined its effects on the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated, murine macrophage-like RAW264.7 cells. Western blot analysis performed with specific anti-iNOS antibodies showed that a decrease in nitric oxide (NO) was accompanied by a decrease in the iNOS protein level. To clarify the mechanistic basis for DPT's ability to inhibit iNOS induction, we examined the effect of DPT on nuclear factor (NF)-kappaB transcriptional activity and DNA binding activity. DPT potently suppressed both reporter gene activity and DNA binding activity. These findings suggest that DPT in RAW264.7 cells abolished LPS-induced iNOS expression by inhibiting the transcription factor, NF-kappaB.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apiaceae / chemistry
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Blotting, Western
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Cell Line
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Chromatography, High Pressure Liquid
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Chromatography, Thin Layer
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DNA / metabolism
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Drugs, Chinese Herbal
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Electrophoresis, Polyacrylamide Gel
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Electrophoretic Mobility Shift Assay
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Indicators and Reagents
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Lipopolysaccharides / antagonists & inhibitors*
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Lipopolysaccharides / toxicity
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Luciferases / genetics
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Macrophages / drug effects
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Macrophages / enzymology
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Macrophages / metabolism*
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Magnetic Resonance Spectroscopy
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Mice
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Nitric Oxide Synthase Type II / biosynthesis*
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Plant Roots / chemistry
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Podophyllotoxin / analogs & derivatives*
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Podophyllotoxin / pharmacology
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Transcription Factor RelA / metabolism*
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Transfection
Substances
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Drugs, Chinese Herbal
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Indicators and Reagents
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Lipopolysaccharides
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Transcription Factor RelA
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deoxypodophyllotoxin
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DNA
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Luciferases
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Nitric Oxide Synthase Type II
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Podophyllotoxin