Objective: To investigate the effect of Guangdong Liangcha Keli on restraint stress-induced liver damage in mice.
Method: Thirty-five male C57BL/6J mice of 7 weeks old were divided into 5 groups randomly with 7 mice in each group: normal group, restraint stress group, 250 mg kg(-1) Vitamin C, Guangdong Liangcha Keli 500 mg kg(-1) and 250 mg kg(-1). After 18 hr restraint stress, the ALT acitivity in plasma, MDA level in plasma and liver, GSH content, GSH-PX and GST activities, NO level and ORAC value in liver were determined.
Result: Compared with restraint model group, Guangdong Liangcha Keli could markedly reduce ALT activity (92.75 +/- 1.91 vs 39.29 +/- 2.56, 32.69 +/- 1.46) U L(-1), and protect the liver damage induced by oxidative stress. In addition, Guangdong Liangcha Keli could effectively increase the ORAC value, GSH content, GSH-PX activity and GST activity and reduce the MDA level and NO level in liver.
Conclusion: Oral treatment of Guangdong Liangcha Keli is found to reduce restraint stress-induced liver damage in terms of above mentioned biochemical parameters, and these protective effects may be related to its free radical scavenging activity and lipid peroxidation inhibitory effects.