Abstract
A continuous-flow porcine cecal bacterial culture has been used experimentally as treatment against enterotoxigenic Escherichia coli in weanling pigs. Periodically, the cultures must be started from frozen stock. Our results indicate that denaturing gradient gel electrophoresis can be applied as an indirect indication of culture similarity for each new batch generated from frozen stock.
MeSH terms
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Animals
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Cecum / microbiology*
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Colony Count, Microbial
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Cryopreservation
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DNA, Bacterial / genetics
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Electrophoresis, Gel, Pulsed-Field / methods*
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Enterotoxigenic Escherichia coli / genetics
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Enterotoxigenic Escherichia coli / isolation & purification*
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Fatty Acids, Volatile / analysis
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Hydrogen-Ion Concentration
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Lactic Acid / analysis
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Probiotics / isolation & purification*
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Swine
Substances
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DNA, Bacterial
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Fatty Acids, Volatile
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Lactic Acid