Abstract
Chlamydophila pneumoniae DNA and mRNA transcripts were investigated by PCR and RT-PCR in fresh CSF and PBMC specimens co-cultured in Hep-2 cell lines and collected from 14 patients with definite RR MS and 19 patients with other inflammatory (OIND) and non-inflammatory (NIND) neurological controls. A positivity for C. pneumoniae DNA and mRNA was detected in CSF and PBMCs of 9 RR MS patients (64.2%) with evidence of disease activity, whereas only 3 controls were positive for Chlamydial DNA. These preliminary findings suggest that C. pneumoniae may occur in a persistent and metabolically active state at both peripheral and intrathecal levels in MS, but not in OIND and NIND.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Causality
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Cerebrospinal Fluid / metabolism
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Cerebrospinal Fluid / microbiology
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Chlamydophila Infections / blood
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Chlamydophila Infections / cerebrospinal fluid
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Chlamydophila Infections / complications*
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Chlamydophila pneumoniae / genetics*
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DNA / analysis
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DNA / blood
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DNA / cerebrospinal fluid
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Encephalitis / blood
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Encephalitis / cerebrospinal fluid
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Female
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Humans
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Leukocytes, Mononuclear / microbiology*
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Male
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Multiple Sclerosis, Relapsing-Remitting / blood
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Multiple Sclerosis, Relapsing-Remitting / cerebrospinal fluid
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Multiple Sclerosis, Relapsing-Remitting / microbiology*
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Nucleic Acids / analysis*
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Nucleic Acids / blood
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Nucleic Acids / cerebrospinal fluid
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Prospective Studies
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RNA, Messenger / blood
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RNA, Messenger / cerebrospinal fluid
Substances
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Nucleic Acids
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RNA, Messenger
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DNA