Abstract
Exopolysaccharides play an important role in the rheology and texture of fermented foods, and among these beta-glucans have immunomodulating properties. We show that the overproduction of the Pediococcus parvulus GTF glycosyltransferase in an uncapsulated Lactococcus lactis strain results in synthesis and secretion (300 mg liter(-1)) of a position 2-substituted (1-->3)-beta-D-glucan that has potential use as a food additive.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Food Microbiology
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Gene Expression
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Genetic Vectors
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Glycosyltransferases / metabolism*
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Lactococcus lactis / enzymology
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Lactococcus lactis / metabolism*
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Pediococcus / enzymology*
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Pediococcus / genetics
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Plasmids
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Polysaccharides, Bacterial / biosynthesis*
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beta-Glucans / isolation & purification
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beta-Glucans / metabolism*
Substances
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Polysaccharides, Bacterial
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beta-Glucans
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Glycosyltransferases