Heterologous expression of a position 2-substituted (1-->3)-beta-D-glucan in Lactococcus lactis

Appl Environ Microbiol. 2008 Aug;74(16):5259-62. doi: 10.1128/AEM.00463-08. Epub 2008 Jun 20.

Abstract

Exopolysaccharides play an important role in the rheology and texture of fermented foods, and among these beta-glucans have immunomodulating properties. We show that the overproduction of the Pediococcus parvulus GTF glycosyltransferase in an uncapsulated Lactococcus lactis strain results in synthesis and secretion (300 mg liter(-1)) of a position 2-substituted (1-->3)-beta-D-glucan that has potential use as a food additive.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Food Microbiology
  • Gene Expression
  • Genetic Vectors
  • Glycosyltransferases / metabolism*
  • Lactococcus lactis / enzymology
  • Lactococcus lactis / metabolism*
  • Pediococcus / enzymology*
  • Pediococcus / genetics
  • Plasmids
  • Polysaccharides, Bacterial / biosynthesis*
  • beta-Glucans / isolation & purification
  • beta-Glucans / metabolism*

Substances

  • Polysaccharides, Bacterial
  • beta-Glucans
  • Glycosyltransferases