To study the biological features of Amblyomma tigrinum, samples of engorged females collected from dogs in the city of Lages, SC, Brazil, were maintained in Petri dishes into an incubator at 27+/-1 degrees C and 80+/-10% RH, for egg laying. For the development of the subsequent experimental phases, artificial infestations were performed in rabbits using the technique of the cotton bag glued to the ear's base. The mean period of hatch, feeding and larval molt were 11.67, 5.93 and 10.96 days, respectively, whereas the mean period of feeding and nymph's molt were 12.40 and 16.27 days, respectively. Only one engorged female was obtained, with detachment observed on the 22(nd) day. The longevity periods for larvae, nymphs and adults were 75, 96 and 150 days respectively.