Five polyphenolic compounds were identified from the diethyl ether and ethyl acetate extracts of the herb of ragged robin, Lychnis flos-cuculi L. (Caryophyllaceae). The identified and isolated compounds were established as a caffeic acid, luteolin, apigenin, apigenin 8-C-beta-D-glucopyranoside (vitexin) and luteolin 8-C-beta-D-glucopyranoside (orientin) by means of extensive spectroscopic studies, including UV and NMR, and confirmed by their chromatographic behavior--TLC and HPLC analyses. This is the first report of occurrence of these compounds in L. flos-cuculi.