Pitx3-CreER mice showing restricted Cre expression in developing ocular lens and skeletal muscles

Genesis. 2008 Jun;46(6):324-8. doi: 10.1002/dvg.20399.

Abstract

To generate temporally controlled inactivation or activation of interested genes in Pitx3-expressing cells, the tamoxifen-inducible form of Cre, CreER(T2), was inserted into the Pitx3 locus of a mouse BAC clone. Following a single dose of tamoxifen, Cre activity in Pitx3-CreER(T2) transgenic mice was observed in the ocular lens and skeletal muscles but not in the central nervous system at various embryonic stages. This mouse line provides a reagent for driving inducible Cre-dependent recombination in the lens and skeletal muscles during embryonic development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromosomes, Artificial, Bacterial / genetics
  • Crosses, Genetic
  • Embryo, Mammalian / metabolism
  • Galactosides / metabolism
  • Gene Expression Regulation, Developmental / drug effects
  • Gene Expression Regulation, Developmental / genetics
  • Gene Targeting / methods
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / metabolism
  • Immunohistochemistry
  • Indoles / metabolism
  • Integrases / genetics*
  • Integrases / metabolism
  • Lens, Crystalline / embryology
  • Lens, Crystalline / metabolism*
  • Mice
  • Mice, Transgenic
  • Muscle, Skeletal / embryology
  • Muscle, Skeletal / metabolism*
  • Organ Specificity / genetics
  • Recombination, Genetic
  • Tamoxifen / administration & dosage
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transgenes

Substances

  • Galactosides
  • Homeodomain Proteins
  • Indoles
  • Transcription Factors
  • homeobox protein PITX3
  • Tamoxifen
  • Cre recombinase
  • Integrases
  • 5-bromo-4-chloro-3-indolyl beta-galactoside