Identification of mutations related to streptomycin resistance in clinical isolates of Mycobacterium tuberculosis and possible involvement of efflux mechanism

Fernanda S Spies; Pedro E Almeida da Silva; Marta O Ribeiro; Maria Lucia Rossetti; Arnaldo Zaha

doi:10.1128/AAC.01570-07

Identification of mutations related to streptomycin resistance in clinical isolates of Mycobacterium tuberculosis and possible involvement of efflux mechanism

Antimicrob Agents Chemother. 2008 Aug;52(8):2947-9. doi: 10.1128/AAC.01570-07. Epub 2008 Jun 9.

Authors

Fernanda S Spies¹, Pedro E Almeida da Silva, Marta O Ribeiro, Maria Lucia Rossetti, Arnaldo Zaha

Affiliation

¹ Programa de Pós-graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

Abstract

The MIC for streptomycin in the presence of efflux pump (EP) inhibitors and the sequencing of rpsL, rrs, and gidB genes provided evidence for the possible participation of EP in low-level streptomycin (STR) resistance of some isolates without mutations. Mutation in the gidB gene and an EP could act synergistically to confer low STR resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
DNA Mutational Analysis
Humans
Microbial Sensitivity Tests
Mutation*
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / metabolism
Streptomycin / pharmacology*
Tuberculosis / microbiology
Tuberculosis, Multidrug-Resistant / genetics*

Substances

Anti-Bacterial Agents
Bacterial Proteins
Streptomycin