The present study examined the association between pain-related anxiety and an on-line interpretation bias for putative physical health threat. Healthy volunteers (n=80) completed measures on Anxiety Sensitivity, Injury/illness Sensitivity, Fear of Pain and Pain Catastrophizing. Furthermore, they performed an interpretation task, in which spontaneous (on-line) inferences were indirectly assessed from reaction times and accuracy of a lexical decision to the final word of an ambiguous description. Results demonstrated a general facilitation of responses to final words that endorsed a health-threatening resolution of ambiguity (e.g., illness). This effect correlated positively with individual levels of Fear of Pain, but was found to be unrelated to levels of Anxiety Sensitivity, Injury/illness Sensitivity or Pain Catastrophizing. Implications of the findings and recommendations for future research are discussed.