Abstract
Using single-molecule polymerase chain reaction, the frequency of spontaneous and radiation-induced mutation at an expanded simple tandem repeat (ESTR) locus was studied in DNA samples extracted from sperm and bone marrow of Atm knockout (Atm(+/-)) heterozygous male mice. The frequency of spontaneous mutation in sperm and bone marrow in Atm(+/-) males did not significantly differ from that in wild-type BALB/c mice. Acute exposure to 1 Gy of gamma-rays did not affect ESTR mutation frequency in bone marrow and resulted in similar increases in sperm samples taken from Atm(+/-) and BALB/c males. Taken together, these results suggest that the Atm haploinsufficiency analysed in our study does not affect spontaneous and radiation-induced ESTR mutation frequency in mice.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Ataxia Telangiectasia Mutated Proteins
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Bone Marrow* / radiation effects
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / physiology*
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DNA Mutational Analysis
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DNA Repeat Expansion / genetics*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology*
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Gamma Rays
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Germ Cells / radiation effects
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Germ-Line Mutation*
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Haploidy
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Male
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Mice
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Mice, Inbred BALB C
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Polymerase Chain Reaction
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / physiology*
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Spermatozoa* / radiation effects
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Tumor Suppressor Proteins / genetics
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Tumor Suppressor Proteins / physiology*
Substances
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Cell Cycle Proteins
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DNA-Binding Proteins
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Tumor Suppressor Proteins
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Ataxia Telangiectasia Mutated Proteins
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Atm protein, mouse
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Protein Serine-Threonine Kinases