Abstract
(S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides. We constructed a system for asymmetric reduction of 2-chloroacrylate to produce (S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/l (S)-2-chloropropionate in more than 99.9%e.e.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acrylates / metabolism*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Burkholderia / enzymology*
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Burkholderia / genetics
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Glucose 1-Dehydrogenase / genetics
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Glucose 1-Dehydrogenase / metabolism*
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Hydrocarbons, Chlorinated
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Oxidation-Reduction
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Propionates / metabolism*
Substances
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2-chloroacrylic acid
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Acrylates
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Bacterial Proteins
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Hydrocarbons, Chlorinated
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Propionates
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2-chloropropionic acid
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Glucose 1-Dehydrogenase