Production of (S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase

J Biosci Bioeng. 2008 Apr;105(4):429-31. doi: 10.1263/jbb.105.429.

Abstract

(S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides. We constructed a system for asymmetric reduction of 2-chloroacrylate to produce (S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/l (S)-2-chloropropionate in more than 99.9%e.e.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylates / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Burkholderia / enzymology*
  • Burkholderia / genetics
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Glucose 1-Dehydrogenase / genetics
  • Glucose 1-Dehydrogenase / metabolism*
  • Hydrocarbons, Chlorinated
  • Oxidation-Reduction
  • Propionates / metabolism*

Substances

  • 2-chloroacrylic acid
  • Acrylates
  • Bacterial Proteins
  • Hydrocarbons, Chlorinated
  • Propionates
  • 2-chloropropionic acid
  • Glucose 1-Dehydrogenase