Abstract
To determine if DNA vaccines for two hantaviruses causing hemorrhagic fever with renal syndrome, Hantaan virus and Puumala virus, are immunogenic when given in combination, we delivered them to hamsters separately or as mixtures by gene gun or by electroporation. Both vaccines elicited neutralizing antibodies when given alone but when they were delivered as a mixture, antibodies to only one of the two hantaviruses could be detected. In contrast, if the DNAs were given as separate vaccinations to a single animal, responses to both were observed. These studies suggest that the two DNA vaccines will need to be given as separate administrations.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antibodies, Viral / blood*
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Biolistics
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COS Cells
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Chlorocebus aethiops
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Cricetinae
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Drug Therapy, Combination
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Electroporation
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Hantaan virus / genetics
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Hantaan virus / immunology*
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Hemorrhagic Fever with Renal Syndrome / immunology
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Hemorrhagic Fever with Renal Syndrome / prevention & control*
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Hemorrhagic Fever with Renal Syndrome / virology
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Neutralization Tests
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Puumala virus / genetics
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Puumala virus / immunology*
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Vaccines, DNA* / administration & dosage
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Vaccines, DNA* / genetics
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Vaccines, DNA* / immunology
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Vero Cells
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Viral Vaccines* / administration & dosage
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Viral Vaccines* / genetics
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Viral Vaccines* / immunology
Substances
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Antibodies, Viral
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Vaccines, DNA
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Viral Vaccines