Determination of anti-tumor agent bis(p-fluorobenzyl)trisulfide and its degraded compound in rat blood using reversed phase high-performance liquid chromatography

Liqiang Gu; Liping Li; Zhongjian Chen; Hao Pan; Huidi Jiang; Su Zeng; Xiao Xu; Haoyun An

doi:10.1016/j.jchromb.2008.04.026

Determination of anti-tumor agent bis(p-fluorobenzyl)trisulfide and its degraded compound in rat blood using reversed phase high-performance liquid chromatography

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jun 1;868(1-2):77-82. doi: 10.1016/j.jchromb.2008.04.026. Epub 2008 Apr 25.

Authors

Liqiang Gu¹, Liping Li, Zhongjian Chen, Hao Pan, Huidi Jiang, Su Zeng, Xiao Xu, Haoyun An

Affiliation

¹ Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.

PMID: 18479981
DOI: 10.1016/j.jchromb.2008.04.026

Abstract

Bis(p-fluorobenzyl)trisulfide (BFTS) demonstrated a broad spectrum of anti-proliferative activity and in vivo anti-tumor efficacy in human xenograft mice models. BFTS is rapidly degraded to its major metabolite bis(p-fluorobenzyl)disulfide (BFDS) in blood. In this study, we developed a reliable procedure for stable storage and treatment of blood samples containing BFTS. An HPLC assay method was developed and validated for the quantitative determination of BFTS and BFDS in rat blood. The developed procedure and method have been successfully utilized to study the concentration-time curves of BFTS and BFDS in the blood of rats after vein injection of 12.5 mg kg(-1) BFTS.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Animals
Antineoplastic Agents / blood*
Chromatography, High Pressure Liquid / methods*
Fluorobenzenes / blood*
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Sulfides / blood*

Substances

Antineoplastic Agents
Fluorobenzenes
Sulfides
fluorapacin