Increased abundance of cytoplasmic and nuclear caveolin 1 in human diploid fibroblasts in H(2)O(2)-induced premature senescence and interplay with p38alpha(MAPK)

FEBS Lett. 2008 May 28;582(12):1685-92. doi: 10.1016/j.febslet.2008.04.026. Epub 2008 Apr 23.

Abstract

Treatment of IMR-90 human diploid fibroblasts with a sublethal concentration of H(2)O(2) induces premature senescence. We investigated the protein abundance, subcellular localization and involvement of caveolin 1 in premature senescence. Caveolin 1 is a scaffolding protein able to concentrate and organize signaling molecules within the caveolae membrane domains. We report the first evidence of increased nuclear and cytoplasmic localization of caveolin 1 during establishment of H(2)O(2)-induced premature senescence. Moreover, we demonstrate that phosphorylation of caveolin 1 during treatment with H(2)O(2) is dependent on p38alpha mitogen-activated protein kinase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Caveolin 1 / genetics
  • Caveolin 1 / metabolism*
  • Cell Nucleus / metabolism*
  • Cellular Senescence*
  • Cytoplasm / metabolism*
  • Diploidy
  • Down-Regulation
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Fibroblasts / physiology
  • Humans
  • Hydrogen Peroxide / toxicity*
  • Mitogen-Activated Protein Kinase 14 / genetics
  • Mitogen-Activated Protein Kinase 14 / metabolism*
  • Oxidative Stress*
  • RNA, Small Interfering / genetics

Substances

  • Caveolin 1
  • RNA, Small Interfering
  • Hydrogen Peroxide
  • Mitogen-Activated Protein Kinase 14