Abstract
After cloning the C3d cDNA of AA broilers using the liver mRNA source, a pair of primers were designed to subclone the P29 gene to the pUC19 plasmid. Several tandems of P29 were constructed in the pUC19 plasmid using a pair of isoschizomers-BamH I and Bgl II. The pUC- P29.n was igested to get the gene of P29.n that was then cloned to pCDNA3.1 (+) plasmid. After this, the F Gene of Newcastle Disease Virus was cloned through RT-PCR and inserted into the upstream of the P29.n that was in the pCDNA-P29.n, and the DNA vaccines containing F gene against NDV with C3d-P29 as molecular adjuvant were constructed. Several groups of Specefic Pathogen Free chickens were injected with these recombinant plasmids. The pCDNA-F-P29.4 and pCDNA-F-P29.6 group had higher HI antibody titers than the pCDNA-F group. The pCDNA-F-P29.4 and pCDNA-F-P29.6 group's HI antibody titers did not achieve titers as high as the inactive vaccine group. However, they all provided protection against the lethal F48E9 virus challenge.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Viral / blood
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Antigens, Helminth / administration & dosage
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Antigens, Helminth / genetics
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Antigens, Helminth / immunology*
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Chickens
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Complement C3d / administration & dosage
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Complement C3d / chemistry
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Complement C3d / genetics
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Complement C3d / immunology*
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Molecular Sequence Data
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Newcastle Disease / genetics
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Newcastle Disease / immunology
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Newcastle Disease / prevention & control*
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Newcastle disease virus / genetics
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Newcastle disease virus / immunology*
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Vaccination
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / genetics
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Vaccines, DNA / immunology
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Viral Fusion Proteins / administration & dosage
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Viral Fusion Proteins / genetics
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Viral Fusion Proteins / immunology
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Viral Vaccines / administration & dosage
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Viral Vaccines / genetics
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Viral Vaccines / immunology*
Substances
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Antibodies, Viral
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Antigens, Helminth
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P-29 antigen
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Vaccines, DNA
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Viral Fusion Proteins
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Viral Vaccines
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Complement C3d