Screening of a Saccharomyces cerevisiae nonessential gene deletion collection for altered susceptibility to a killer peptide

Stefania Conti; Walter Magliani; Laura Giovati; Irene Libri; Domenico Leonardo Maffei; Antonella Salati; Luciano Polonelli

Screening of a Saccharomyces cerevisiae nonessential gene deletion collection for altered susceptibility to a killer peptide

New Microbiol. 2008 Jan;31(1):143-5.

Authors

Stefania Conti¹, Walter Magliani, Laura Giovati, Irene Libri, Domenico Leonardo Maffei, Antonella Salati, Luciano Polonelli

Affiliation

¹ Dipartimento di Patologia e Medicina di Laboratorio, Sezione di Microbiologia, University of Parma, Parma, Italy.

PMID: 18437854

Abstract

Approximately 4,800 Saccharomyces cerevisiae mutants deleted for nonessential genes were screened for alterations in susceptibility to a synthetic killer peptide (KP). None of the tested strains, including mutants resistant to conventional antifungal drugs, showed increased or decreased susceptibility to KP in comparison with the parental strain. The results may reflect the peculiar mechanism of action of KP and claim the possible avoidance of vital resistant mutants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Deletion*
Killer Factors, Yeast
Microbial Sensitivity Tests
Mutation
Mycotoxins / chemistry*
Peptides / chemical synthesis*
Peptides / chemistry
Peptides / pharmacology*
Saccharomyces cerevisiae / drug effects*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / genetics*
Saccharomyces cerevisiae Proteins / metabolism

Substances

K1 killer toxin
Killer Factors, Yeast
Mycotoxins
Peptides
Saccharomyces cerevisiae Proteins