Abstract
Thin-layer chromatography analysis revealed that the contents stored in oil bodies isolated from jelly fig (Ficus awkeotsang Makino) achenes were mainly neutral lipids (>90% triacylglycerols and approximately 5% diacylglycerols). Fatty acids released from the neutral lipids of achene oil bodies were highly unsaturated (62.65% alpha-linolenic acid, 18.24% linoleic acid, and 10.62% oleic acid). The integrity of isolated oil bodies was presumably maintained via electronegative repulsion and steric hindrance provided by their surface proteins. Immunological cross-recognition using antibodies against sesame oil-body proteins indicated that two oleosin isoforms and one caleosin were present in these oil bodies. MALDI-MS analyses confirmed that the three full-length cDNA fragments obtained by PCR cloning from maturing achenes encoded the two jelly fig oleosin isoforms and one caleosin identified by immunological screening.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Blotting, Western
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Chromatography, Thin Layer
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Diglycerides / chemistry
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Diglycerides / metabolism
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Electrophoresis, Polyacrylamide Gel
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Fatty Acids / chemistry
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Fatty Acids / metabolism
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Ficus / genetics
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Ficus / metabolism*
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Inclusion Bodies / chemistry
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Inclusion Bodies / metabolism*
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Linoleic Acid / chemistry
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Linoleic Acid / metabolism
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Molecular Sequence Data
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Oleic Acid / chemistry
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Oleic Acid / metabolism
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Plant Oils / chemistry
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Plant Oils / metabolism*
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Plant Proteins / chemistry
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Polymerase Chain Reaction
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Seeds / genetics
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Seeds / metabolism*
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Sequence Homology, Amino Acid
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Triglycerides / chemistry
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Triglycerides / metabolism
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alpha-Linolenic Acid / chemistry
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alpha-Linolenic Acid / metabolism
Substances
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Diglycerides
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Fatty Acids
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Plant Oils
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Plant Proteins
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Protein Isoforms
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Triglycerides
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alpha-Linolenic Acid
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Oleic Acid
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Linoleic Acid
Associated data
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GENBANK/EF583922
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GENBANK/EF583923
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GENBANK/EU157185