Abstract
A protocol for a fluorescent intercalator displacement (FID) assay useful for establishing DNA binding selectivity, affinity, stoichiometry, and binding site size, and for distinguishing modes of DNA binding is presented.
MeSH terms
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Binding Sites
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DNA / chemistry*
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DNA / metabolism*
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Ethidium / chemistry
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Ethidium / metabolism
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism
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Intercalating Agents / chemistry*
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Intercalating Agents / metabolism
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Oligodeoxyribonucleotides / chemistry
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Oligodeoxyribonucleotides / metabolism
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Spectrometry, Fluorescence / methods*
Substances
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Fluorescent Dyes
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Intercalating Agents
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Oligodeoxyribonucleotides
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DNA
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Ethidium