Abstract
A one-pot synthesis of homodimeric proteins is described. The synthetic strategy is based on a double expressed protein ligation reaction between thioester peptides and a new bis-cysteinyl linker. The protocol was also applied to the synthesis of heterodimers.
MeSH terms
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Chromatography, High Pressure Liquid / methods
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Cross-Linking Reagents / chemistry
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Dimerization
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Ligands
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Molecular Structure
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Peptides / chemistry
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Proteins / chemical synthesis*
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Proteins / chemistry
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Sulfhydryl Compounds / chemical synthesis
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Sulfhydryl Compounds / chemistry
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Time Factors
Substances
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Cross-Linking Reagents
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Ligands
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Peptides
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Proteins
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Sulfhydryl Compounds